Purification and Properties of the Bifunctional Proline Dehydrogenase/1-Pyrroline-5-Carboxylate Dehydrogenase from Pseudomonas aeruginosa
نویسندگان
چکیده
منابع مشابه
Isolation, Purification and Characterization of Proline Dehydrogenase from a Pseudomonas putida POS-F84 Isolate
The purpose of this study was to isolate and characterize Proline Dehydrogenase (ProDH) enzyme frommicroorganisms isolated from soil in Iran. Isolation and screening of L-proline degradative enzymes from soilsamples was carried out. The isolate was characterized by biochemical markers and 16S rRNA geneanalysis. The target ProDH was purified and the effects of pH and temperatur...
متن کاملPurification, characterization, and crystallization of human pyrroline-5-carboxylate reductase.
Pyrroline-5-carboxylate reductase (P5CR) catalyzes the reduction of Delta1-pyrroline-5-carboxylate (P5C) to proline with concomitant oxidation of NAD(P)H to NAD(P)(+). The enzymatic cycle between P5C and proline is very important in many physiological and pathological processes. Human P5CR was over-expressed in Escherichia coli and purified to homogeneity by chromatography. Enzymatic assays of ...
متن کاملPurification to homogeneity of pyrroline-5-carboxylate reductase of barley.
An enzyme has been purified to homogeneity from barley seedlings which has ;proline dehydrogenase' and the pyrroline-5-carboxylic acid reductase activities. The purification achieved is 39,000-fold as calculated from the proline dehydrogenase activity. The subunit molecular weight of the protein is 30 kilodaltons. The native enzyme has molecular weights up to 480 kilodaltons, depending on the b...
متن کاملPyrroline-5-carboxylate reductase of Neurospora crassa; partial purification and some properties.
Previous studies (1, 2) with mutant and wild type strains of Neurospora crassa have pointed to glutamic y-semialdehyde and its spontaneously cyclized form, Al-pyrroline-5-carboxylate, as intermediates in the biosynthesis of proline. These studies, based largely on experiments in vivo, have recently been substantiated through work in vitro with extracted enzymes (3, 4). ‘The terminal step in pro...
متن کاملA bifunctional enzyme (delta 1-pyrroline-5-carboxylate synthetase) catalyzes the first two steps in proline biosynthesis in plants.
Many plants synthesize and accumulate proline in response to osmotic stress. Despite the importance of this pathway, however, the exact metabolic route and enzymes involved in the synthesis of proline in plants have not been unequivocally identified. We report here the isolation of a mothbean (Vigna aconitifolia) cDNA clone encoding a bifunctional enzyme, delta 1-pyrroline-5-carboxylate synthet...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: European Journal of Biochemistry
سال: 1982
ISSN: 0014-2956,1432-1033
DOI: 10.1111/j.1432-1033.1982.tb07021.x